FIG. 5.

HYPK interacts specifically with hNaa15p of hNatA, not with hNaa25p of hNatB and hNaa35p of hNatC. HEK293 cells were cotransfected with plasmids encoding Xpress-HYPK plus hNaa15p-V5, hNaa25p-V5, hNaa35p-V5, or lacZ-V5, as indicated. At 48 h posttransfection, cells were harvested and subjected to immunoprecipitation using anti-Xpress (anti-Xp) antibodies. The presence of hNaa15p-V5, hNaa25p-V5, and hNaa35p-V5 in complex with Xpress-HYPK was investigated by SDS-PAGE and Western blotting, using anti-V5. (Top) Western blot of immunoprecipitates. (Bottom) Western blot of cell lysates prior to immunoprecipitation. The results shown are representative of three independent experiments.