FIG. 2.

PR-B mediates induction of E2F1 expression by R5020. (A and B) The indicated T47D:C42 cells were synchronized and treated with vehicle (veh) or 10 nM R5020 for 18 (A) or 16 (B) h. wt, wild type. (C) Human mammary epithelial cells (HMECs) were infected with a β-gal (negative control)- or PR-B-expressing adenovirus and subsequently treated with vehicle or 10 nM R5020 for 16 h. (A to C) After treatment, cells were lysed and RNA was isolated and reverse transcribed. S100P or E2F1 mRNA levels were quantified using qPCR and normalized to the housekeeping gene 36B4. Results are expressed as mean fold induction over vehicle-treated cells ± SEM (n = 3).