FIG. 7.

OGFOD1 regulates survival of arsenite-induced stress. (A) Knockdown of OGFOD1 reduces accumulation of the 89-kDa PARP cleavage product in cells recovering from arsenite-induced stress. Cells were treated with a negative-control siRNA (neg. cntrl.) and siRNAs against G3BP1 or OGFOD1 72 h prior to harvest. Extracts were prepared from unstressed cells (untreated), arsenite-treated cells, and cells recovering from arsenite-induced stress. Proteins were analyzed by Western blotting. (B) Overexpression of OGFOD1 increases accumulation of the 35-kDa caspase-9 and 89-kDa PARP cleavage products in cells exposed to arsenite. Extracts were prepared as described above from HeLa cells transiently transfected with an empty DNA vector (neg. cntrl.) or with plasmids expressing G3BP1 and OGFOD1 cDNAs and subjected to Western blot analysis.