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. 2010 Feb 12;30(8):2006–2016. doi: 10.1128/MCB.01350-09

FIG. 9.

FIG. 9.

OGFOD1 and HRI interact with eIF2α. (A) HRI colocalizes with G3BP1 in arsenite-induced stress granules. Endogenous HRI and G3BP1 were detected by indirect immunofluorescence in untreated HeLa cells or in cells treated with 1 mM arsenite for 30 min at 37°C. An overlap of HRI and G3BP1 appears white in the merged image. White arrows point to stress granules. (B) OGFOD1 band 2 coimmunoprecipitates with HRI. Extracts from untreated or arsenite-treated HeLa cells were subjected to immunoprecipitation with an antibody against HRI. Beads without added antibody and Hif1α immunoprecipitation were used as controls for nonspecific interactions. Proteins were separated by SDS-PAGE and detected by Western blot analysis using the indicated antibodies. (C) OGFOD1 and HRI coimmunoprecipitate eIF2α and phosphorylated eIF2α. Extracts from untreated or arsenite-treated HeLa cells were subjected to immunoprecipitation with antibodies against OGFOD1 and HRI. Beads without added antibody and Hif1α immunoprecipitation were used as controls for nonspecific interactions. Proteins were separated by SDS-PAGE and detected by Western blot analysis using the indicated antibodies.