FIG. 3.

Reversibility of pH-induced conformational changes in gB. Extracellular HSV-1 KOS virions were treated with medium buffered to pH 7.2 or 5.5. For the indicated samples, pH was neutralized back to 7.2 for 10 min at 37°C. Twofold dilutions were blotted immediately to nitrocellulose membranes. Membranes were probed at neutral pH with antibody H126, DL16, or R69, followed by the appropriate horseradish peroxidase-conjugated secondary antibody. The exposures shown document the reversibility of reactivity.