FIG. 3.

Wild-type E1A affects the removal of corepressor complexes from the promoters of cyclin A and Cdc6, whereas the E1A.928 mutant cannot. (A and B) Quiescent wild-type E1A-inducible cells were treated with or without Dox for the indicated times, and afterward ChIP analyses were performed using antibodies specific for p130, E2F4, HDAC1, and mSin3B. Control reactions were performed with NR IgG and anti-MyoD. Bottom row in panel A represents PCR analysis with GAPDH primers on input chromatin (0.05% of total) and confirms the use of equivalent amounts of chromatin in each of the ChIP reactions. (C) Quiescent mutant E1A.928-inducible cells were treated with Dox for 12 h, and afterward ChIP assays were performed using anti-p130, anti-E2F4, anti-HDAC1, and anti-mSin3B antibodies. A control reaction was performed in parallel with anti-MyoD antibody.