FIG. 3.

Insertion of an IE62 consensus site lowers transactivation of the gI promoter in transfection and superinfection experiments. (A) Sequences of the native (top) and mutated (bottom) gI promoter regions. Underlining indicates the positions of the wild-type partial consensus site, the mutated full consensus site, and the atypical gI promoter TATA sequence ATAAA. (B) Results of reporter assays in MeWo cells transfected with 1 μg of the pgI-Luc and pgI-Luc/ATCGT reporter plasmids and 0.01 μg, 0.02 μg, or 0.04 μg of pCMV62. (C) Results of reporter assays in MeWo cells transfected with 1 μg of the pgI-Luc and pgI-Luc/ATCGT reporter plasmids followed by superinfection with VZV-infected cells 24 h following the initial transfection. The luciferase activity from the reporter in the absence of infection or cotransfection was normalized to 1, and experimental activities are reported as n-fold activation over this level.