FIG. 5.

Tandem consensus sites exacerbate the inhibitory effect seen on IE62 transactivation of a model TATA promoter in transfection experiments. (A) Schematic showing position of the IE62 consensus site relative to the TATA box in the model promoter constructs. (B) Results of reporter assays. One microgram of pTA-Luc, p10US-Luc, p2xUS-Luc, or p3xUS-Luc reporter plasmid was cotransfected with 0.01 μg, 0.02 μg, or 0.04 μg of pCMV62 into MeWo cells. The luciferase activity from the reporters in the absence of infection or cotransfection was normalized to 1, and experimental activities are reported as n-fold activation over this level. (C) Results of reporter assays in MeWo cells transfected with 1 μg of the reporter plasmids followed by superinfection with VZV infected cells 24 h following the initial transfection. The luciferase activity from the reporter in the absence of infection or cotransfection was normalized to 1, and experimental activities are reported as n-fold activation over this level.