FIG. 9.

Mutation of a native consensus site increases IE62-mediated transactivation of the gC promoter. (A) Position of the IE62 consensus site relative to the gC TATA box. One microgram of wild-type or mutant reporter plasmid was transfected into MeWo cells with 0.01 μg, 0.02 μg, or 0.04 μg of the pCMV62 expression plasmid (C) or followed by superinfection 24 h posttransfection (B). The luciferase activity from the reporters in the absence of infection or cotransfection was normalized to 1, and experimental activities are reported as n-fold activation over this level.