FIG. 6.
Inhibition of R8102 infection by gHt.st/gL. Cells were preincubated with gHt.st/gL at the indicated concentrations for 1 h. R8102 (3 PFU/cell) was added to the gHt.st/gL-containing medium for 90 min of incubation. The viral inoculum was removed, and cells were overlaid with gHt.st/gL and processed for β-Gal quantification at 8 h after infection. The negative controls consisted of incubation with heat-inactivated gHt.st/gL for 120 min at 80°C (heat-gHt.st/gL) or medium alone. A 100% infection value corresponds to infected cells not exposed to gHt.st/gL. 293T cells were either left untransfected or transfected with αV and β3 integrin plasmids (293TαVβ3). J and CHO cells were transfected either with nectin1 alone (J-N1 and CHO-N1) or with nectin1 plus αV and β3 integrin plasmids (J-N1αVβ3 and CHO-N1αVβ3). Each point represents the average of triplicate assays. Each experiment was performed at least two times. Infection is expressed as a percentage of the highest value obtained for each panel.