TABLE 1.
Replication of recombinant Newcastle disease virus strains in normal human and tumor cells
| Cell line | Virus yield (PFU/ml)a |
|
|---|---|---|
| rBC | rBC-Edit | |
| SVHUC1 (normal human uroepithelial) | <10 | <10 |
| PC3 (prostate cancer) | 2.0 × 106 | 2.1 × 104 |
| HT1080 (fibrosarcoma) | 1.0 × 106 | 1.0 × 104 |
| HuTu80 (adenocarcinoma) | 3.0 × 1011 | 1.0 × 108 |
| THP-1 (monocytic leukemia) | 1.0 × 103 | <10 |
| CCRF-CEM (T cell lymphoblast-like) | <10 | <10 |
| HeLa (cervical cancer) | 1.9 × 107 | 1.9 × 107 |
| HEpG2 (hepatocarcinoma) | 1.5 × 107 | 4.8 × 106 |
| CaCo2 (colon cancer) | 7.3 × 1010 | 3.6 × 108 |
| CoLo205 (colon cancer) | 1.5 × 104 | 1.0 × 104 |
| HT29 (colon cancer) | 5.0 × 104 | 1.0 × 104 |
| T84 (colon cancer) | 1.0 × 105 | 1.2 × 105 |
| SH-SY5Y (neuroblastoma) | 7.5 × 105 | 5.0 × 106 |
| MCF-7 (breast cancer) | 1.5 × 103 | 3.0 × 102 |
a
Cells were infected at an MOI of 0.01 with rNDV, and virus yield was assessed from culture supernatants at 48 h p.i. by plaque assay on DF1 cells.