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Characterization of EYFP-tagged fusion loop mutants. (a) Surface expression of EYFP-tagged gB fusion loop mutants in fixed, nonpermeabilized cells. (b) Quantification of fusion levels in cells triggered with soluble gD306 (luciferase assay). (c) gB fusion loop mutants and wild-type gB do not interact with gH/gL in the absence of gD. (d) Wild-type gB interacts with gH/gL, an interaction that leads to fusion. (e to g) Fusion loop mutants are negative for both BiMC and fusion.
