FIG. 2.

Dose titration analysis for WT and mutant CD317 in cells coexpressing WT or Vpu-defective HIV-1. Titration of plasmids encoding HA-CD317, CD317, or CT mutants of HA-CD317 in 293T cells cotransfected with either pHIV-1 wt or pHIV-1Δvpu. Culture supernatants were analyzed for the yield of infectious HIV-1 two days posttransfection. Shown are the levels of infectious secreted virus relative to WT HIV-1 in the absence of CD317 (empty vector, set to 100%). Values depicted are the arithmetic means of triplicates ± SD from one experiment representative of two experiments.