FIG. 4.

Infection of CE(NiN) strain inhibits activation of IRF-3-dependent but not NF-κB-dependent IFN-β promoter. SYM-I cells were cotransfected with pRL-TK and pNF-κB-Luc (NF-κB-responsive reporter plasmid) (A) or 4×IRF-3-Luc (IRF-3-responsive reporter plasmid) (B). After 24 h, the cells were mock infected or infected with each strain at an MOI of 2. The luciferase activities were measured 24 h after transfection. The data represent firefly luciferase activity normalized to Renilla luciferase activity and are presented as means (± the SD) of three independent replicates. *, Significant difference (P < 0.01); ns, no significant difference.