FIG. 9.

CE(NiN) strain efficiently replicates in cells overexpressing RIG-I, but not RIG-IN. 293T cells were transfected with 0.05, 0.1, and 1 μg of pEF-Flag-RIG-I (A) or pEF-Flag-RIG-IN (C). After 24 h, cells were infected with Ni-CE and CE(NiN) strains at an MOI of 3. At 24 hpi, the cells were lysed and N protein, tubulin, RIG-I and RIG-IN were detected by Western blotting. (B and D) Ratio of each N protein band to its tubulin control was calculated by using ImageJ. Panels B and D show the results of experiments using pEF-Flag-RIG-I (A) and pEF-Flag-RIG-IN (C), respectively. Each bar represents the mean (± the SD) of three independent replicates. *, Significant difference versus mock-transfected cells (P < 0.01).