TABLE 2.
Lysate batch size (no. of lysates) [with controls included] | No. (%) of unused aliquots per master mix vial following reconstitution |
||
---|---|---|---|
8 aliquots | 9 aliquots | 10 aliquots | |
1 [3] | 5 (62.5) | 6 (66.7) | 7 (70.0) |
2 [4] | 4 (50.0) | 5 (55.6) | 6 (60.0) |
3 [5] | 3 (37.5) | 4 (44.4) | 5 (50.0) |
4 [6] | 2 (25.0) | 3 (33.3) | 4 (40.0) |
5 [7] | 1 (12.5) | 2 (22.2) | 3 (30.0) |
6 [8] | 0 (0.0) | 1 (11.1) | 2 (20.0) |
Mean % of unused aliquots per random distribution batch size | 31.3 | 38.9 | 45.0 |
Mandated controls are added to batch size prior to the calculation of unused reaction tubes.