TABLE 2.
Characterization of 185 invasive macrolide-resistant S. pneumoniae strains
| Phenotype (no. of isolates) and STa | ST(s) found in this study | Related PNEM clone(s) | No. of isolates (%) | Gene(s) detected by PCRb | Presumed transposon(s) (no. of isolates)b | Serotype(s) (no. of isolates) |
|---|---|---|---|---|---|---|
| MLSB (168) | ||||||
| ST81 | ST81, ST3708 | Spain23F-1 | 24 (14.3) | erm(B), tet(M), int, xis | Tn6002 (17) | 23F (13), 19F (4) |
| erm(B), tet(M), int, xis, tnpA, tnpR | Tn3872 (6) | 19A (5), 19F (1) | ||||
| erm(B), tet(M), tndX | Tn5397* (1) | 19A (1) | ||||
| ST63 | ST63, ST2100, ST3997 | Sweden15A-25 | 24 (14.3) | erm(B), tet(M), tndX | Tn5397 (23) | 15A (10), 19F (7) 15F (3), 19A (2), 15B (1) |
| erm(B)† | None (1) | 15A (1) | ||||
| ST230 | ST230, ST276, ST2307, ST3980 | Denmark14-32 | 19 (11.3) | erm(B), tet(M), int, xis | Tn6002 (14) | 24F (11), 19A (2), 19F(1) |
| erm(B), tet(M), aph3′, int, xis | Tn1545/Tn6003 (3) | 19A (3) | ||||
| erm(B), tet(M), tndX | Tn5397* (1) | 24F (1) | ||||
| erm(B), tet(M), int, xis, tnpA, tnpR | Tn3872 (1) | 19A (1) | ||||
| ST90 | ST90, ST1624, ST3996 | Spain6B-2 | 15 (8.9) | erm(B), tet(M), int, xis | Tn6002 (12) | 6B (11), 6A (1) |
| erm(B), tet(M), int, xis, tnpA, tnpR | Tn3872 (2) | 6B (2) | ||||
| erm(B), tet(M), aph3′, int, xis | Tn1545/Tn6003 (1) | 6B (1) | ||||
| ST15 | ST9, ST17, ST1201, ST1664, ST3988 | England14-9, Spain14-5 | 12 (7.1) | erm(B), tet(M), int, xis, tnpA, tnpR | Tn3872 (8) | 14 (5), 19A (3) |
| erm(B), tet(M), int, xis | Tn6002 (2) | 6A (1), 19A (1) | ||||
| erm(B)† | None (2) | 6B (1), 14 (1) | ||||
| ST87 | ST88, ST87 | 8 (4.8) | erm(B), tet(M), int, xis, tnpA, tnpR | Tn3872 (4) | 19F (4) | |
| erm(B), tet(M), int, xis | Tn6002 (3) | 19F (3) | ||||
| erm(B), tet(M), tndX | Tn5397* (1) | 19F (1) | ||||
| ST156 | ST156 | Spain9V-3 | 8 (4.8) | erm(B), tet(M), int, xis | Tn6002 (3) | 14 (2), 9V (1) |
| erm(B), tet(M), int, xis, tnpA, tnpR | Tn3872 (3) | 14 (3) | ||||
| erm(B), tet(M), aph3′, int, xis | Tn1545/Tn6003 (1) | 14 (1) | ||||
| erm(B), mef(E), tet(M), tndX | MEGA+Tn5397* (1) | 19A (1) | ||||
| ST311 | ST42 | 8 (4.8) | erm(B), tet(M), int, xis | Tn6002 (6) | 23A (5), 23F (1) | |
| erm(B), tet(M), int, xis, tnpA, tnpR | Tn3872 (1) | 23A (1) | ||||
| erm(B), tet(M), tndX | Tn5397* (1) | 23A (1) | ||||
| ST717 | ST717 | 8 (4.8) | erm(B), tet(M), int, xis | Tn6002 (8) | 33F (7), 3 (1) | |
| ST315 | ST315 | Poland 6B-20 | 5 (3.0) | erm(B), tet(M), int, xis | Tn6002 (5) | 6B (4), 6A (1) |
| ST271 | ST202, ST271, ST320 | 7 (4.2) | erm(B), tet(M), int, xis, tnpA, tnpR | Tn3872 (5) | 19A (4), 19F (1) | |
| erm(B), mef(E), tet(M), int, xis | Tn2010 (2) | 19A (1), 19F (1) | ||||
| ST2591 | ST3977, ST3987, ST3998 | 4 (2.4) | erm(B), tet(M), aph3′, int, xis | Tn1545/Tn6003 (2) | 6A (2) | |
| erm(B), tet(M), int, xis | Tn6002 (2) | 6A (2) | ||||
| ST260 | ST260 | 3 (1.8) | erm(B), tet(M), int, xis | Tn6002 (1) | 3 (1) | |
| erm(B), tet(M), int, xis, tnpA, tnpR | Tn3872 (2) | 3 (2) | ||||
| ST66 | ST67 | Tennessee14-18 | 3 (1.8) | erm(B), tet(M), int, xis | Tn6002 (3) | 14 (3) |
| ST30 | ST30 | 3 (1.8) | erm(B), tet(M), int, xis | Tn6002 (3) | 16F (3) | |
| ST177 | ST179 | Portugal19F-21 | 2 (1.2) | erm(B), tet(M), int, xis, tnpA, tnpR | Tn3872 (2) | 19F (2) |
| ST304 | ST304 | Sweden1-40 | 2 (1.2) | erm(B), tet(M), int, xis | Tn6002 (2) | 1 (2) |
| ST460 | ST97 | 1 (0.6) | erm(B), tet(M), int, xis | Tn6002 (1) | 10 (1) | |
| ST135 | ST135 | 1 (0.6) | erm(B), tet(M), int, xis, tnpA, tnpR | Tn3872 (1) | 6B (1) | |
| ST191 | ST191 | Netherlands7F-39 | 1 (0.6) | erm(B), tet(M), int, xis | Tn6002 (1) | 7F (1) |
| ST344 | ST344 | NorwayNT-42 | 1 (0.6) | erm(B), tet(M), int, xis, tnpA, tnpR | Tn3872 (1) | 19A (1) |
| ST306 | ST306 | Sweden1-28 | 1 (0.6) | erm(B)† | None (1) | 1 (1) |
| ST386 | ST386 | 1 (0.6) | erm(B), tet(M), int, xis | Tn6002 (1) | 6A (1) | |
| ST558 | ST558 | 1 (0.6) | erm(B)† | None (1) | 35B (1) | |
| Not founder | ST1866, ST3986, ST3989 | 3 (1.8) | erm(B), tet(M), int, xis, tnpA, tnpR | Tn3872 (1) | 4 (1) | |
| erm(B), tet(M), int, xis | Tn6002 (2) | 6A (1), 23A (1) | ||||
| MLST not done | 3 (1.8) | erm(B), tet(M), int, xis | Tn6002 (2) | 15A (1), NT (1) | ||
| erm(B), tet(M), int, xis, tnpA, tnpR | Tn3872 (1) | NT (1) | ||||
| M (17) | ||||||
| ST156 | ST156 | Spain9V-3 | 9 (52.9) | mef(E)† | MEGA (9) | 14 (9) |
| ST176 | ST138, ST3979 | 2 (11.8) | mef(E)† | MEGA (1) | 6B (1) | |
| mef(A)† | Tn1207.1 (1) | 6A (1) | ||||
| ST15 | ST9 | England14-9 | 1 (5.9) | mef(A)† | Tn1207.1 (1) | 14 (1) |
| ST30 | ST30 | 1 (5.9) | mef(E)† | MEGA (1) | 16 (1) | |
| ST63 | ST3978 | Sweden15A-25 | 1 (5.9) | mef(E)† | MEGA (1) | 14 (1) |
| ST242 | ST242 | Taiwan23F-15 | 1 (5.9) | mef(E), tet(M), int, xis | Tn2009 (1) | 23F (1) |
| ST473 | ST473 | 1 (5.9) | mef(E)† | MEGA (1) | 6A (1) | |
| MLST not done | 1 (5.9) | mef(E)† | MEGA (1) | NT (1) |
a
ST, that is, the predicter founder in the global MLST database.
b
*, Tn5397-related element with expression of tet(M) gene; †, tetracycline-susceptible strains.