FIG. 1.
PFGE profiles of the three clinical strains of Globicatella from the patient and the type strains of G. sanguinis and G. sulfidifaciens. Patterns were obtained by SmaI macrorestriction, with a method adapted from that of van den Braak et al. (12). Lanes: M, molecular size markers (48.5 to 970 kb [size standard no. 170-3635; Bio-Rad]); 1, G. sanguinis strain CIP 107044T; 2, G. sulfidifaciens strain CIP 107175T; 3, strain HDP50220 (isolated from CSF); 4, strain HDP60616 (isolated from the groin); and 5, strain HDP60617 (isolated from the rectum). According to the criteria of Tenover et al., strain HDP50220 and strain HDP60616 are closely related, as their profiles differ by only three DNA fragments (10). These patterns differed from the pattern of the rectal strain and from the individual patterns of the type strains.