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PCR assay validation. (A) Mean CT values (double reactions) of plasmid dilutions containing 107 to 102 genome equivalents of pYam and pVic were plotted against the cycle number. The slope and correlation (R2) are indicated. (B) Probit analyses were performed by examination of plasmid dilutions containing 100 to 0.1 genome equivalents of pYam and pVic in 10-fold reactions. Results were analyzed by using SPSS 17.0 statistics software.
