FIG. 3.
(A) Plasmid localization of the blaOXA-58 gene in A. baumannii ST2/A and ST78/B isolates. Agarose (1%) gel electrophoresis in 1× Tris-acetate-EDTA buffer of HindIII-digested plasmids from A. baumannii isolates 3979 and 3957, respectively, stained with ethidium bromide and visualized under UV light and Southern blot hybridization with the blaOXA-58 probe are shown. Lane M shows a 1-kb DNA ladder (Promega, Milan, Italy). (B) Schematic map of the genetic structure surrounding the blaOXA-58 gene in A. baumannii ST2/A and ST78/B isolates. The genes and their corresponding transcription orientations are indicated by horizontal arrows. IS elements are represented by open rectangles filled with black arrows indicating the transposase gene and the direction of transcription. Names of relevant features are reported below or above the map.