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(A) Linearity of the pan-Aspergillus NASBA assay determined by triplicate amplification of total nucleic acids extracted from serially diluted A. fumigatus conidia with 105 to 101 spores. (B) Linearity of Aspergillus qPCR assay. The same TNA series used for the experiment whose results are presented in panel A were used to generate the standard curve.
