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Aspergillus burdens in lung tissues (A) and BAL fluids (B) in terms of CFU counting, qPCR, and real-time NASBA measurements. UI, uninfected group. A linear increase in the Aspergillus burden in lung tissues was observed by PCR (F = 40.66, P < 0.0001) and NASBA (F = 141.10, P < 0.0001).