TABLE 1.
Aspergillus burden in lung tissue and BAL fluid measured by CFU, qPCR, and real-time NASBA assays
| Sample type | Time (h) postinfection (no. of rats) |
Aspergillus burden determined bya: |
|||||
|---|---|---|---|---|---|---|---|
| Culture |
qPCR |
NASBA |
|||||
| No. of positive samples | Mean log10 CFU/g (ml)b | No. of positive samples | Mean log10 CE/g (ml)b | No. of positive samples | Mean log10 CE/g (ml)b | ||
| Lung tissue | 1 (5) | 5 | 5.17 ± 0.25 | 5 | 3.07 ± 0.25 | 0 | |
| 24 (5) | 5 | 3.80 ± 0.44 | 5 | 3.50 ± 0.39 | 5 | 2.87 ± 0.61 | |
| 48 (5) | 5 | 3.70 ± 0.50 | 5 | 3.66 ± 0.67 | 4 | 3.92 ± 0.88 | |
| 72 (5) | 5 | 4.00 ± 0.64 | 5 | 4.23 ± 0.66 | 5 | 5.10 ± 0.77c | |
| 96 (5) | 5 | 3.75 ± 0.62 | 5 | 5.46 ± 0.76c | 5 | 6.00 ± 0.78c | |
| BAL fluid | 1 (5) | 5 | 3.20 ± 0.51 | 2 | 2.66 ± 0.47 | 3 | 2.48 ± 0.17 |
| 24 (5) | 3 | 2.44 ± 0.28 | 5 | 4.01 ± 0.97c | 5 | 4.37 ± 0.53c | |
| 48 (5) | 2 | 2.24 ± 0.09 | 4 | 4.55 ± 1.54c | 5 | 4.13 ± 1.16c | |
| 72 (5) | 0 | 3 | 3.40 ± 0.38 | 4 | 3.99 ± 0.45c | ||
| 96 (5) | 3 | 1.70 ± 0.00 | 2 | 3.20 ± 1.37 | 5 | 3.37 ± 1.01c | |
a
Mean log10 ± standard deviation was used to describe the Aspergillus burden.
b
Mean log10 CFU/g and mean log10 CE/g are units for lung tissue burden measurement, and mean log10 CFU/ml and mean log10 CE/ml are units for BAL fluid burden assessment.
c
Statistically significant difference (P < 0.05) compared to culture CFU counts.