Skip to main content
. 2010 Feb 10;48(4):1231–1240. doi: 10.1128/JCM.01767-09

TABLE 1.

Summary of methods used to test the 2008 EAPCRI WB panela

Center Vol (ml) used RCL WCL Fungal lysis Method of fungal DNA purification DNA strategy Internal control PCR No. of replicates Compliant PCR strategy
2 3 Yes Yes B. beating QIAamp Blood kit (Qiagen) DNA 1 No 3 No PCR 1
3 3 Yes No B. beating MagNA Pure LC Total NA kit (Roche) DNA 2 Yes 3 Yes PCR 2
4 1-2 Yes No Lyticase Phenol-chloroform DNA 3 Yes 1 No PCR 3
5 3 Yes Yes Lyticase DNAeasy Tissue (Qiagen) DNA 4 No 3 No PCR 4
6 3 Yes No B. beating MagNA Pure LC DNA kit I (Roche) DNA 5 No 3 No PCR 5
7 3 Yes No B. beating ZR Fungal/Bacterial DNA kit (Zymo research) DNA 6 Yes 3 Yes PCR 6
8 1.5 Yes No B. beating SeptiFast kit (Roche) DNA 7 Yes 3 No PCR 7
9 3 Yes Yes B. beating SeptiFast kit (Roche) DNA 8 Yes 3 Yes PCR 7
10 3 Yes Yes B. beating MagNA Pure LC Total NA kit (Roche) DNA 9 Yes 3 Yes PCR 1
11 3 Yes Yes B. beating Tissue kit, EZ1 (Qiagen) DNA 10 Yes 3 Yes PCR 8
13 3 Yes Yes B. beating High Pure Template PCR kit (Roche) DNA 11 Yes 3 Yes PCR 1
14 3 Yes Yes B. beating SeptiFast kit (Roche) DNA 8 Yes 3 Yes PCR 9
15 3 No No B. beating QIAamp blood kit (Qiagen) DNA 12 Yes 3 Yes PCR 1
16b 3 ND ND B. beating QIAamp DNA minikit (Qiagen) DNA 13 Yes 3 Yes PCR 10
17 3 Yes Yes Lyticase DNA Lego kit (Top Bio) DNA 14 No 3 No PCR 11
18 3 Yes No B. beating MagNA Pure LC DNA kit I (Roche) DNA 5 No 3 No PCR 5
19 3 Yes Yes B. beating GeneXpert (Cepheid) DNA 15 Yes 3 Yes PCR 12
20 3 Yes Yes B. beating High Pure Template PCR kit (Roche) DNA 11 Yes 1 No PCR 2
21 2 Yes No B. beating SeptiFast kit (Roche) DNA 7 Yes 3 No PCR 1
22 3 Yes Yes B. beating High Pure Template PCR kit (Roche) DNA 11 Yes 3 Yes PCR 13
23 3 Yes Yes B. beating bioMérieux MiniMagc DNA 16 Yes 3 Yes PCR 1
a

The results for the centers that used methods that attained the detection threshold are indicated in boldface. These included 11 compliant centers and 3 noncompliant centers. Eleven different DNA extraction techniques were used together with nine different PCR protocols by the 14 centers attaining the threshold, 9 of which were compliant; 8 attained the PCR performance threshold, and 5 achieved enhanced PCR performance. No results were received from centers 1 and 12, whereas logistical limitations prohibited the participation of center 24. Abbreviations: RCL, red cell lysis; WCL, white cell lysis; B. beating, bead beating; ND, not disclosed.

b

The specificity of the assay was poor (11.1%).

c

For the extraction reagents, any possible contaminating DNA sources were removed by magnetic silica treatment.