TABLE 5.
Positivity rates for the two EAPCRI WB panels and PCR performance panela
| Fungal load (total no. of conidia) | Potential total no. of rRNA copiesb | No. of copies/μlc | Positivity rate (% [no. of PCR tests]) |
||
|---|---|---|---|---|---|
| PCR panel (n = 52) | 2007 panel (n = 61) | 2008 panel (n = 59) | |||
| 2.7 × 105 | 94.2 (49) | NT | NT | ||
| 2.7 × 104 | 94.2 (49) | NT | NT | ||
| >10,000 | 5.4 × 105 | 5.4 × 103 | NT | 77.0 (47) | NT |
| 2.7 × 103 | 92.3 (48) | NT | NT | ||
| 1,000 | 5.4 × 104 | 5.4 × 102 | NT | 75.4 (46) | 98.3 (58) |
| 500 | 2.7 × 104 | 2.7 × 102 | 94.2 (49) | 57.4 (35) | 98.3 (58) |
| 100 | 5.4 × 103 | 5.4 × 101 | NT | 34.4 (21) | 78.0 (46) |
| 75 | 4.1 × 103 | 4.1 × 101 | NT | 36.1 (22) | 67.8 (40) |
| 50 | 2.7 × 103 | 2.7 × 101 | 86.5 (45) | 37.7 (23) | 74.6 (44) |
| 20-25 | 1.1-1.4 × 103 | 1.1-1.4 × 101 | NT | 29.5 (18) | 62.7 (37) |
| 10 | 5.4 × 102 | 5.4 | NT | 27.9(17) | 49.2 (29) |
| 2.7 | 40.4 (21) | NT | NT | ||
| 0d | 0 | 0 | 2.9 (3)e | 4.9 (6)f | 11.3 (20)g |
A total of 22 centers contributed results for the PCR performance panel and the 2007 WB panel, although 1 center returned results only for the PCR panel, while another returned results only for the WB panel. For the 2008 WB panel, 21 centers returned the results. The theoretical threshold is indicated in boldface. NT, not tested in this panel.
Based on 54 copies of rRNA copies per genome (9).
The calculations were based on 100% extraction efficiency and an elution volume of 100 μl.
The 1st panel contained two negative control specimens. The 2nd panel contained three negative control specimens.
As the panel contained two negative specimens, the total number of tests was 104 and not 52.
For one center, both negative specimens were PCR positive. As the panel contained two negative specimens, the total number of tests was 122 and not 61.
As the panel contained three negative specimens, the total number of tests was 177 and not 59.