TABLE 1.
Targets, primers, and probes for the various vaccine MGB assays with associated working concentrationsa
| Vaccine | Target (position) | Gene description | Working concn (nM) of: |
|
|---|---|---|---|---|
| Probe | Primer | |||
| B. abortus S19 | BruAB1_0060 (290)b | Transcription regulator (lysR family) | VAC: 5′-CCGATAGGAGAACCG-3′ (150) | F: 5′-ACGCTCTGGATCGCATATTCATC-3′ (900) |
| NON: 5′-CCGATAGGCGAACCG-3′ (100) | R: 5′-GCACAGATCGGCGAACTG-3′ (100) | |||
| B. abortus S19 | BruAB1_1114 (450) | Chaperone (clpX) | VAC: 5′-CTGACGCAAGCCG-3′ (250) | F: 5′- CACATCTTCGCCGACATAGC-3′ (700) |
| NON: 5′-CTGACGGAAGCCG-3′ (50) | R: 5′-CATCGATGTGCCGTTCATCATG-3′ (100) | |||
| B. abortus RB51 | BAB1_0649 (667)b | Glutathione S- transferase | VAC: 5′-CTCCTCTTCCGTATTGG-3′ (250) | F: 5′-TGCTGGTTGGCAAGGAAGAG-3′ (100) |
| NON: 5′-CTCCTCTTCCTTATTGG-3′ (50) | R: 5′- CGATCAACGCGCCTTCTG-3′ (700) | |||
| B. melitensis Rev 1 | BMEI0752 (271)b | Ribosomal protein S12 (rpsL) | VAC: 5′-ACACCCAGCAAGTC-3′ (250) | F: 5′-TGACACCCTGGGTATCGAGAA-3′ (700) |
| NON: 5′-ACACCCGGCAAGTC-3′ (50) | R: 5′-GTGGCGGTCGTGTGAAG-3′ (100) | |||
| B. melitensis Rev 1 | BMEI0208 (933)b | Gamma-glutamyl phosphate reductase (proA) | VAC: 5′-CGCAGGTTTTGCC-3′ (50) | F: 5′-CGCGTCGAGATATTCGGTTGA-3′ (900) |
| NON: 5′-CGCAGGCTTTGCC-3′ (250) | R: 5′-GGTGCTGGCGCTCTATCC-3′ (100) | |||
a
The position of the SNP within each target is shown in parentheses. The target SNP is shown underlined and bold within the vaccine (VAC) and nonspecific (NON) probes.
b
Probes based on the reverse complement of the target SNP region.