TABLE 1.

Results of real-time PCR using BHrecA, IS481, and IS1001 for patient specimens collected over a 2-year period^a

Target	Organism detected	2007 specimens			2008 specimens
		No. of specimens	% of total Bordetella-positive specimens	% of total specimens	No. of specimens	% of total Bordetella-positive specimens	% of total specimens
IS481	B. pertussis	883	95.4	17.7	824	97.1	15.6
IS1001	B. parapertussis	26	2.8	0.5	18	2.1	0.3
IS481 and IS1001	B. pertussis and B. parapertussisb	8	0.9	0.2	4	0.5	0.5
IS481 and BHrecA	B. holmesii	9c	1.0	0.2	3d	0.4	0.1
Total Bordetella positive		926		18.6	849		16.5
Total specimens received		4,983			5,271

^aBordetella species were differentiated based on the detection of IS481 without detection of BHrecA (B. pertussis) or detection of both IS481 and BHrecA (B. holmesii).

^bCoinfection.

^cSix were culture positive.

^dTwo were culture positive.