Western blot analysis of sera from patients with CD or other parasitic diseases, probed with anti-CttFbn peptide sera. (A) Analysis of 28 CD-negative control sera shows that the C-terminally truncated fragment of Fbn (28.9 kDa) was detected in only 3/28 sera (11%). (B) Analysis of 28 CD-positive sera shows that the 28.9-kDa band was detected in 22/28 sera (79%). Fbn, control recombinant Fbn fragment; CD+, pool of 8 positive Chagasic sera; CD−, pooled Canadian negative sera. Lanes in which the 28.9-kDa band was present are marked by asterisks. (C) Analysis of pools of sera from patients with fasciolosis (F), schistosomiasis (S), malaria (M), leishmaniasis (L), African trypanosomiasis (A), or Chagas' disease (CD+) or from Canadian negative controls (CD−). The antipeptide serum detected a band near 28.9 kDa only in the CD+ serum pool.