TABLE 2.
Primer sequences for amplification of the 23S rRNA gene
| Primer name | Sequence | Positiona |
|---|---|---|
| 23sFtul_1nf | 5′-ATG AAG GAC GTG ATA ATC TGC G-3′ | 56-76 |
| 23sFtul_2r | 5′-AAC CTC CTG GCT GTC TGG G-3′ | 1022-1040 |
| 23sFtul_2f | 5′-TGT ATA ACT CAT TGG GGT AAA GC-3′ | 821-842 |
| 23sFtul_3r | 5′-TAC CCA CCA TAC ACT GTC CTC-3′ | 2188-2200 |
| 23sFtul_3f | 5′-GAA GTA TAG GGT GTG ACG CC-3′ | 1788-1807 |
| 23sFtul_4r | 5′-CAC ACC CAG CCT ATC AAC G-3′ | 2792-2810 |
Positions designate the localization of the primers within a consensus sequence of the 23S rRNA gene of the genus Francisella.