TABLE 2.
Results of HBV genotyping of 185 samples also genotyped by sequencing or RFLP
| Sequencing/RFLP genotype (no. of samples)a | No. (%) of samples with TaqMan genotypeb: |
||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| A | B | C | D | E | F | G | H | Only Q probe | Nondetectable | Mixed A/D | |
| A (24) | 23 (96) | 1 | |||||||||
| B (26) | 26 (100) | ||||||||||
| C (38) | 35 (92) | 3c | |||||||||
| D (79) | 77 (97) | 1 | 1 | ||||||||
| E (8) | 7 (88) | 1 | |||||||||
| F (2) | 2 (100) | ||||||||||
| G (1) | 1 (100) | ||||||||||
| Hd (1) | 1 (100) | ||||||||||
| X/C recombinante (6) | 6f | ||||||||||
a
A total of 148 samples were identified by RFLP (19 of them were also sequenced, with congruent results), and 36 were genotyped only by sequencing.
b
Numbers in parentheses are percentages of the corresponding number of samples genotyped by sequencing or RFLP.
c
Subgenotype C5 strains (by sequencing).
d
Genotype H was represented by a pUC57 plasmid carrying a synthetic segment of the pre-S/S region of HBV genotype H.
e
The X/C recombinant strain, first described by Hannoun et al. (9), is made up partly by genotype C DNA and partly by untypeable DNA.
f
Two of these six samples also showed weak reactivity with the AS set, but with CT values >10 cycles higher than for Q.