Figure 4.

SHC1disruption in NSCLC cell lines and clinical tumors. (a) Representative array CGH profiles for samples with and without SHC1 amplification. Vertical lines denote log₂ signal ratios from −1 to 1 with copy number increases to the right (red lines) and decreases to the left (green lines) of 0 (purple line), with amplified region shaded orange. Red and green arrows mark clones used in subsequent Fluorescence in situ hybridization (FISH) analysis. (b) SHC1 expression in NSCLC cell lines. The normalized fold change of expression compared to a normal lung reference is plotted for samples with amplification (red) and those with neutral copy number status (black). (c) Overexpression of SHC1 in clinical tumors. The log₂ fold change in expression levels of SHC1 relative to their matched normal lung tissue is plotted for each tumor. The P-value from the Wilcoxon sign-rank test is indicated. (d and e) FISH confirmation of SHC1 amplification in H1395. FISH was performed using BAC clones mapping to SHC1 (RP11-624P9) and to an adjacent neutral copy number region (RP11-313J15).