Figure 3.

Conditional deletion of itgβ8 from postmitotic neurons using nex-cre does not result in cerebral hemorrhage. A, P0 brain lacking itgβ8 in postmitotic neurons because of conditional deletion mediated by nex-cre. B, C, Nissl-stained sections of a nex-cre mutant P0 brain. D, F, LacZ-stained sections from an E14.5 embryo cortex and P0 cortex obtained from a cross between a nex-cre-positive animal and the R26R reporter line. Cre-mediated recombination is very strong in the future cortical plate by E14.5 when hemorrhage is observed in the nestin-cre mutant and remains strong after birth. E, β-Galactosidase (βGal) expression analysis of E14.5 embryo cortex. Note that βGal (green) immunolabeling is limited to cells within the future cortical plate. Endothelial cells were immunolabeled with isolectin B4 (red) and were not recombined by this cre line. Cell nuclei were counterstained with TO-PRO-3 (blue). Scale bar: E, 50 μm.