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. 2005 Oct 26;25(43):9940–9948. doi: 10.1523/JNEUROSCI.3467-05.2005

Figure 4.

Figure 4.

Conditional deletion of itgβ8 in the neuroepithelium leads to endothelial cell irregularities in the P0 neonatal cortex. Anti-PECAM immunolabeling of P0 coronal sections of the forebrain to visualize vascular endothelial cells is shown. A, B, Control. F, G, Nestin-cre-targeted mutant. K, L, Tie2-cre-targeted mutant. Notice the bulbous organization of endothelial cell clusters in the cortex (arrows) of the nestin-cre-targeted mutant (F, G). Anti-collagen IV immunolabeling of P0 coronal sections of the neuroepithelium to visualize the basal lamina surrounding the vasculature is shown. C, E, Control. H, J, Nestin-cre-targeted mutant. M, O, Tie2-cre-targeted mutant. Notice the intact basement membrane in the area of an endothelial cell cluster (arrowhead) in the nestin-cre-targeted mutant (H, J). Anti-α-smooth muscle actin (αSMA) immunolabeling of pericytes in P0 coronal sections of the neuroepithelium. D, E, Control. I, J, Nestin-cre mutant. N, O, Tie2-cre mutant. Note the normal recruitment of pericytes to the vasculature in both mutants. Scale bars: A, 80 μm; B, E, 40 μm.