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. Author manuscript; available in PMC: 2010 Apr 5.
Published in final edited form as: Biochemistry. 2009 Jul 28;48(29):7056–7071. doi: 10.1021/bi900697c

Figure 2.

Figure 2

EPR spectra of Fe(III)Rev-erbβ(LBD) (A) and Fe(III)E75(LBD) (B). A) X-band EPR spectrum of Fe(III)Rev-erbβ(LBD). The sample was ~180 µM heme in 50 mM borate with 500 mM NaCl, pH 8.0. The spectrum was recorded at 10 K, 9.3580 GHz microwave frequency, 0.319 mW microwave power, 6.3 × 105 receiver gain, 8.31 G modulation amplitude, 100 kHz modulation frequency, 163.84 ms time constant, using 8 added scans each containing 2048 data points. The background was corrected and three sets of g values (a), (b) and (c) were simulated. B) X-band EPR spectrum of Fe(III)E75(LBD). The sample was approximately 130 µM heme in 50 mM borate with 500 mM NaCl, pH 8.0. The spectrum was recorded at 10 K, 9.3588 GHz microwave frequency, 0.636 mW microwave power, 6.3 × 105 receiver gain, 8.31 G modulation amplitude, 100 kHz modulation frequency, 163.84 ms time constant, using 10 added scans each containing 2048 data points. The background was corrected and two sets of g values (a) and (b) were simulated.