Skip to main content
. Author manuscript; available in PMC: 2010 Apr 5.
Published in final edited form as: Biochemistry. 2009 Jul 28;48(29):7056–7071. doi: 10.1021/bi900697c

Figure 4.

Figure 4

Electronic absorption (A) and MCD (B) spectra of Fe(II)Rev-erbβ(LBD). A) The electronic absorption spectrum at 25 °C of Fe(II)Rev-erbβ(LBD) after the addition of solid sodium dithionite to Fe(III)Rev-erbβ(LBD) (thin gray line) is shown. The scan immediately following reduction (black dotted line) undergoes a blue shift to the final spectrum (black line) after 3–5 minutes. The sample contained ~10 µM heme in 25 mM EPPS with 500 mM NaCl, pH 8.0. B) The MCD sample was ~25 µM heme in 25 mM with 250 mM NaCl, pH 8.0, ~55% glycerol. Reduction was performed using solid sodium dithionite. The averages of three scans at 4.5, 8, 15 and 25 K at constant magnetic field (7 tesla) are shown.