Table 1.
Comparison of electronic absorption and MCD peak positions for low-spin, 6-coordinate Fe(III) heme proteins. The experimental values for Rev-erbβ (LBD) and E75(LBD) are shaded.
| Electronic Absorption | ||||||||
|---|---|---|---|---|---|---|---|---|
| Protein | Ligands | δ | Soret | β | α | LMCT | Ref. | |
| Rev-erbβ (LBD) | X/Cys | 359 | 420 | 541 | 563 | 664, 757 | a | |
| E75(LBD) | X/Cys | 360 | 423 | 542 | 569 | 650, 750 | a | |
| E75(261) | N-,O-,S-?/Cysn | 359 | 424 | 542 | 575 | NRb | (23) | |
| RrCooA | Pro/Cys | 362 | 424 | 540 | 574 | 649,750 | (46) | |
| P450CAM+ImH | ImH/Cys | 358 | 425 | 542 | 574 | 638,753 | (73) | |
| hCBS | His/Cys | 365 | 428 | 550 | NR | 650,700 | (35) | |
| M80C cyt c | His/Cys | 355 | 416 | 540 | 570 | 635,734 | (73) | |
| MCD | Peak | Crossover | Trough | Peak | Crossover | Trough | Ref. | |
| Rev-erbβ(LBD) | X/Cys | 404 | 412 | 427 | 562 | 571 | 579 | a |
| E75(LBD) | X/Cys | 414 | 421 | 429 | NRb | NRb | NRb | a |
| RrCooA | Pro/Cys | 413c | 420 | 427c | 562c | 571 | 580b | (47) |
| P450CAM+ImH | ImH/Cys | 417 | 426 | 435 | 563 | 569 | 585 | (74) |
| hCBSc | His/Cys | 419 | 425 | 433 | 545 | 555 | 567 | (35) |
| M80C cyt c | His/Cys | 415c | 424c | 432 b | MSd | 560c | MSd | (74) |
a
This work.
b
Not reported.
c
Negligible intensity.
d
Peak and/or crossover positions were not reported in the original paper and are presented from inspection of the data.
e
Multiple max/crossover/min signals are present; only that of the central crossover position is indicated.