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. Author manuscript; available in PMC: 2010 Sep 1.
Published in final edited form as: Dev Neurobiol. 2009 Sep 1;69(10):633–646. doi: 10.1002/dneu.20732

Figure 6.

Figure 6

Sema 3A treatment reduces the proportion of cortactin and Arp-2 staining relative to total protein. (A) Untreated growth cones showing cortactin staining (green) compared to staining for total protein (red). (B) Following Sema 3A treatment (bath application of 500 ng/ml for 30 min) cortactin staining is reduced compared to total protein in the residual growth cone and connecting neurite. (C) Quantitative comparison of the ratio of cortactin staining to total protein. The decrease (*) was significant (P=0.0002, s.e.m.=0.14, 0.04, N=40). B, Bar= 8 µm. (D) Sema 3A treatment reduces the proportion of Arp-2 staining relative to total protein. Untreated growth cones showing Arp-2 staining (green) compared to staining for total protein (red). (E) Following Sema 3A treatment (bath application of 500 ng/ml for 30 min) Arp-2 staining is reduced compared to total protein in the residual growth cone and connecting neurite. (F) Quantitative comparison of the ratio of Arp-2 staining to total protein. The decrease (*) was significant (P=0.0001, s.e.m.= 0.06, 0.03 N= 40 for each). E, Bar= 8 µm.