Table 1.
Set of oligonucleotide primers (F, forward; R, reverse) used to amplify 11 microsatellite loci of Plasmodium falciparum
(The fluorescent dyes used to label primers were 6-FAM (6-carboxyfluorescein, ‘blue’ label), VIC (Applied Biosystems proprietary ‘green’ fluorescent dye), and NED (Applied Biosystems proprietary ‘yellow’ fluorescent dye).)
| Name | 5′ Label | Sequence 5′–3′ | GenBank Accession number | Chromosome |
|---|---|---|---|---|
| Polyα-F | AAAATATAGACGAACAGA | G37809 | 4 | |
| Polyα-R | VIC | ATCAGATAATTGTTGGTA | ||
| TAA60-F | VIC | CTCAAAGAAAAATAATTCA | G38876 | 13 |
| TAA60-R | AAAAAGGAGGATAAATACAT | |||
| ARA2-F | NED | GTACATATGAATCACCAA | G37848 | 11 |
| ARA2-R | GCTTTGAGTATTATTAATA | |||
| PfG377-F | GATCTCAACGGAAATTAT | G37851 | 12 | |
| PfG377-R | NED | TTATGTTGGTACCGTGT | ||
| PfPK2-F | CTTTCATCGATACTACGA | G37852 | 12 | |
| PfPK2-R | NED | CCTCAGACTGAAATGCAT | ||
| TAA87-F | VIC | ATGGGTTAAATGAGGTACA | G38838 | 6 |
| TAA87-R | ACATGTTCATATTACTCAC | |||
| TAA109-F | 6-FAM | TAGGGAACATCATAAGGAT | G38842 | 6 |
| TAA109-R | CCTATACCAAACATGCTAAA | |||
| TAA81-F | 6-FAM | GAAGAAATAAGGGAAGGT | G38836 | 5 |
| TAA81-R | TTTCACACAACACAGGATT | |||
| TAA42-F | VIC | ACAAAAGGGTGGTGATTCT | G38832 | 5 |
| TAA42-R | GTATTATTACTACTACTAAAG | |||
| 2490-F | TTCTAAATAGATCCAAAG | G37790 | 10 | |
| 2490-R | 6-FAM | ATGATGTGCAGATGACGA | ||
| C2M3-F | GGTTAATATGATCACAAAATG | G37900 | 2 | |
| C2M3-R | NED | ATTGTTGATTCATGAAATGCA |