Large scale N-glycan analysis during P19CL6 cell differentiation. A, MALDI-TOF-MS of whole N-glycans of undifferentiated and differentiated cells. IS, internal standard. B, quantitative and total glycomics of undifferentiated and differentiated cells. C, magnification to visualize increased glycans (*, p < 0.01; **, p < 0.05). D, magnification to visualize decreased glycans (*, p < 0.01; **, p < 0.05). Error bars mean standard deviations. E, bar coding analysis. Intens., intensity; a.u., arbitrary units.