Table 4.
Analytical recovery and imprecision data for quantification of Δ9-tetrahydrocannabinol (THC), 11-hydroxy-THC (11-OH-THC), 11-nor-9-carboxy-THC (THCCOOH), cannabidiol (CBD), and cannabinol (CBN) in oral fluid.
| Imprecision | ||||
|---|---|---|---|---|
| Analytes | Target (ng/mL) | Inter-assay (mean %RSD,N=20, 5 replicates, 4 assays) | Intra-assay (%RSD, N=6) | Analytical recovery (N=20) |
| CBD | 1.5 | 2.8 | 1.1 | 112.4 |
| 7.5 | 2.2 | 1.2 | 109.2 | |
| 30 | 2.6 | 0.6 | 104.3 | |
| THC | 1.5 | 3.0 | 2.2 | 111.1 |
| 7.5 | 2.4 | 1.0 | 105.8 | |
| 30 | 2.5 | 0.9 | 103.8 | |
| CBN | 1.5 | 2.8 | 2.6 | 107.9 |
| 7.5 | 3.6 | 2.1 | 99.1 | |
| 30 | 6.6 | 1.8 | 100.8 | |
| 11-OH-THC | 1.5 | 3.0 | 0.3 | 112 |
| 7.5 | 4.6 | 0.9 | 106.6 | |
| 30 | 3.7 | 0.8 | 103.6 | |
| THCCOOHa | 0.015 | 4.3 | 5.2 | 105.9 |
| 0.075 | 2.3 | 1.4 | 113.8 | |
| 0.3 | 3.6 | 3.2 | 107.8 | |
a
Data from two-dimensional gas chromatography mass spectrometry (2D-GCMS) with negative chemical ionization; all other data from 2D-GCMS with electron ionization.
*
%RSD - percent relative standard deviation.