. Author manuscript; available in PMC: 2010 Apr 5.

Published in final edited form as: Mol Cell Biochem. 2009 Jan 10;324(1-2):39–53. doi: 10.1007/s11010-008-9983-2

Table 1. Accessibility of W⁶⁰¹ and W⁶⁶⁹ to collisional fluorescence quencher.

Values are the Stern-Volmer quenching constants (K_D) obtained as described under “Materials and Methods”.

Quencher	W⁶⁰¹		W⁶⁶⁹		NATA
	Native	Denatured ^a	Native	Denatured ^a

Acrylamide	4.0	7.8	5.5	7.1	24.5

Proteins were denatured by incubation in a buffer containing 6 M guanidine-HCl for 3 min.

Titration with acrylamide followed the denaturation step.

Table 1. Accessibility of W601 and W669 to collisional fluorescence quencher.