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. Author manuscript; available in PMC: 2010 Aug 1.
Published in final edited form as: Dev Cell. 2009 Aug;17(2):222–233. doi: 10.1016/j.devcel.2009.06.010

Figure 5. PAR-1 does not affect signaling of a Mib-independent form of Dll1.

Figure 5

(A, B) Embryos were injected with indicated RNAs and processed for in situ hybridization with N-tubulin probe as described in Fig. 1. RNAs were injected at 0.3 ng. Dorsal views are shown, with anterior to the left, the injected side is up (light blue). Arrows point to changes in N-tubulin expression. (A) PAR-1 suppressed the inhibitory effect of untagged Dll1 on neurogenesis, but had no effect on the activity of Dll1ΔC-Ub.

(B) Quantification of PAR-1 effects on Dll1 and Notch signaling. Numbers of embryos per group are indicated above bars. Data are pooled from several independent experiments. RNA doses are indicated per embryo.

(C) PAR-1 does not affect Notch reporter activation by Dll1ΔC-Ub. Signal-sending cells were transfected with PAR-1, Dll1 or Dll1ΔC-Ub constructs, and cocultured with signal-receiving cells that were transfected with Notch and pGL3-11CSL-Luc, as described in Fig. 2D. *, p<0.01, and #, p<0.01, indicate significant differences from control- or the Dll1-transfected group, respectively. Results are presented as the means ± s. d. of three independent experiments, each carried out with triplicate samples per group.