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Design of PPP1CC2-rescue transgenic construct and genotyping transgenic mice. A) The Pgk2-Ppp1cc2 cDNA-SV40 poly A (pA) transgene. B) An RT-PCR was used to detect the presence of transgenic mRNA of expected length (1.3 kb) in Ppp1cc2-rescue testis. M, marker; R, rescue; −, negative control.
