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. 2009 May 6;81(2):343–352. doi: 10.1095/biolreprod.109.076398

FIG. 2.

FIG. 2.

The presence of PPP1CC2 in rescue mouse testis. A) Transgenic PPP1CC2 is restricted to testis and is not expressed in brain. B) The amount of PPP1CC2 expressed in each rescue line testis is estimated by Western blot analysis: E-line rescue mice express approximately one third of wild-type (WT) PPP1CC2 expression; D-line rescue mice, approximately one sixth; A-line rescue mice, approximately one seventh; and G-line rescue mice, approximately one fifteenth. C) Concentration curve/Western blot comparing E-line rescue mice with wild-type protein expression. D) Immunohistochemistry of wild-type, Ppp1cc−/− (Null), and E-line (Rescue) testes. Sections were probed with rabbit anti-PPP1CC2 antibody, followed by goat anti-rabbit Cy3-labeled secondary antibody. Slides were viewed with the FluoView 500 fluorescence microscope at 20× magnification.