FIG. 6.

The effect of an adenylyl cyclase stimulator in combination with PDE inhibitors on COC cAMP levels. The cAMP was measured in COCs after 4 h of IVM either after no stimulation or after forskolin (100 μM) treatment alone or in combination with rolipram (10 or 100 μM) or dipyridamole (10, 50, or 250 μM). The data represent the mean cAMP level per COC ± SEM of four replicates. Each measurement was conducted on 10 COCs. Different lowercase letters represent a statistical difference between the means as determined by ANOVA followed by Dunnett post hoc test.