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. 2009 Apr 15;81(2):258–266. doi: 10.1095/biolreprod.108.075200

FIG. 1.

FIG. 1.

Temporal and cellular expression of ETV5 in WT testes. The expression of Etv5 mRNA by real-time PCR in neonatal testes (A) was approximately half of that in adult (120-day-old) brain, which has the highest Etv5 mRNA level in mice, and is comparable to that in adult testis. Results are the mean ± SEM. Values with different lowercase letters are significantly different from each other (P < 0.05); n = 4 for each time point except the adult brain sample. B–D) Immunohistochemistry for ETV5 in WT testis at Fetal Day 16 (FD16) and Postnatal Days (P) 0 and 4. B) At FD16, ETV5 expression in the seminiferous cords was limited to Sertoli cell nuclei (Sc), whereas the gonocytes (G−) were negative. Some interstitial cells (Is) were also positive. C and D) In contrast to fetal testis, the pattern of expression was different at P0 and P4, when Sertoli cells and some gonocyte nuclei (G+) showed positive staining, but other gonocytes were negative (G−). Bar = 25 μm.