Figure 10.

GLT1a immunoreactivity within hippocampal terminals and astrocytes of P7 mice. The tissue was obtained from +/+ (A, B) and –/– (C) littermates of a GLT1+/– × GLT1+/– cross. A shows two profiles immunolabeled with the anti-cGLT1a antibody, identifiable as terminals (T) by the presence of a few vesicles (small arrows). Both A and B show immunolabeled profiles identifiable as astrocytes by the irregular contours of their plasma membranes (arrowheads). Both images were taken from the stratum radiatum of the CA1. In A, a putative postsynaptic specialization is marked with an open arrow. The LM results from the same tissue are shown in Figure 4, which also shows images obtained from age-matched knock-out littermates. C shows GLT1a immunoreactivity in a knock-out (–/–) littermate. The tissue was sampled from the CA3 region of the hippocampus. White arrowheads point to the tissue surface in which exposure to immunoreagents is expected to be the greatest and demonstrate that this section was, in fact, taken from this region of the block. Scale bar (in B): A, B, 500 nm; C, 500 nm.