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. Author manuscript; available in PMC: 2011 May 25.
Published in final edited form as: Virology. 2010 Mar 5;401(1):49–60. doi: 10.1016/j.virol.2010.01.020

Fig. 8. H5 is required for IV formation and maturation of IV to MV.

Fig. 8

Cultures of BSC40 cells were infected with either wt (IHDW) or mutant (Dts57, tsH5–4) viruses at the 31°C in the presence of rifampicin. At 12 h p.i., rifampicin was washed out of the cells and shifted up to the non-permissive temperature (SHIFT/RIF RELEASE; right column) or cells were maintained in rifampicin at the permissive temperature (RIF; left column) for an additional 24 h. Characteristic rifampicin bodies are seen in the left column. Arrows indicate flaccid membranes surrounding the virosomes; D DNA crystalloids. Top plate; right column: Normal intermediates of morphogenesis are observed in wt infections: v virosomes; IV immature virions; MV mature virions. Middle plate; right column: Dts57 infections cannot progress beyond IV formation: v virosomes; IV immature virions. Bottom plate; right column: tsH5-4 infections are arrested at IV formation. Clearing of the cytoplasm with empty IVs and crescents (arrow) were observed with curdled virosomes (CV) and few dense IVs in the periphery of the clearing.

HHS Vulnerability Disclosure