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. Author manuscript; available in PMC: 2011 Apr 1.
Published in final edited form as: Mech Dev. 2010 Jan 4;127(3-4):183–202. doi: 10.1016/j.mod.2009.12.003

Figure 3. Fstl1 Transcript Expression in Cellular Fractions of WAT and in Adipogenesis of Primary Preadipocytes.

Figure 3

A. Fstl1 transcript in stromal vascular fraction (SVF) and adipocyte fraction (AF) of murine WAT. Northern blot shows transcript level for SMAF1, SCD1, Retn and Fstl1. B. Q- PCR assessment of Fstl1 transcript level in cell fractions of WAT. cDNA derived from SVF or adipocyte fraction (AF) of subcutaneous (SC) or epididymal (EP) WAT was used for Q-PCR analysis of Fstl1 transcript. Note: the third column represents SC tissue but the white color of the bar cannot be discerned due to low column height. The level in SC AF was set to 1. * indicates p<0.001 for SC SVF vs. SC AF and for EP SVF vs. EP AF. # indicates p<0.001 for EP SVF vs. SC SVF. C. Fstl1 transcript downregulation during adipogenic conversion of rat primary white preadipocytes. RNA was collected from preadipocytes (0) and at indicated times post-induction of adipocyte differentiation and analyzed by Northern blot using murine Fstl1, SCD1 and SMAF1 cDNA probes. D. Fstl1 transcript level in a panel of adult murine tissues. 5 µg of total RNA from indicated tissues were analyzed by Northern blot using murine Fstl1 cDNA probe. For A, C and D, EtBr staining of rRNA is shown as a gel loading control.