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. Author manuscript; available in PMC: 2011 Mar 1.
Published in final edited form as: J Pharmacol Toxicol Methods. 2010 Jan 22;61(2):136–145. doi: 10.1016/j.vascn.2010.01.001

Table 1.

Summary of Current Organotypic Methods for Ocular Toxicology Testing

Name* Validation Status Test Method Indicator Objective of Test
HET-CAM Evaluated; limited usefulness Hemorrhage, coagulation, cytolysis Ocular sensitivity & corrosion
BCOP Validated by ICCVAM for ocular corrosives and severe irritants Increase in corneal opacity, thickness and permeability Ocular sensitivity & corrosion
ICE Validated by ICCVAM for ocular corrosives and severe irritants Increase in corneal opacity, thickness and permeability Ocular sensitivity & corrosion
IRE Evaluated; limited usefulness Increase in corneal opacity and thickness Ocular sensitivity & corrosion
EpiOcular™ (MatTek) In validation studies (ECVAM) MTT cell viability Ocular sensitivity & corrosion
HCE™ (SkinEthic) In validation studies (ECVAM) MTT cell viability Ocular sensitivity & corrosion
*

Abbreviations: BCOP = bovine corneal; HCE = human corneal epithelium; HET-CAM = hen's egg test-chorioallantoic membrane; ICE = isolated chicken eye; IRE = isolated rabbit eye; MTT = methylthiazol tetrazolium test.